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KMID : 0545120150250111902
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Journal of Microbiology and Biotechnology
2015 Volume.25 No. 11 p.1902 ~ p.1907
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Isolation of the Inositol Phosphoceramide Synthase Gene (AUR1) from Stress-Tolerant Yeast Pichia kudriavzevii
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Yoo Boung-Hyuk
Kim Myoung-Dong
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Abstract
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This study is the first report of the entire nucleotide sequence of an inositol phosphoceramide synthase gene from the stress-tolerant yeast Pichia kudriavzevii (PkAUR1). Sequence analysis revealed an open reading frame that spans 1,443 bp and encodes a 480-amino-acid-residue protein with the highest sequence similarity (41.7%) to Aur1 from Spathaspora passalidarum. A phenotypic assay with transformed S. cerevisiae and P. kudriavzevii indicated that two amino acid residues, Phe166 and Gly249, play crucial roles in the resistance to aureobasidin A, which is consistent with previous reports for other fungal Aur1s. The GenBank Accession No. for PkAUR1 is KP729614.
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KEYWORD
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Pichia kudriavzevii, inositol phosphoceramide synthase, AUR1, Aureobasidin A, Site-directed mutagenesis
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